Role of the TrkC receptor in the progress of multiple myeloma - CoMET Laboratory - Poitiers

​

Multiple myeloma is a bone marrow pathology in which an accumulation of plasma cells occurs in the bone marrow, preventing the normal development and production of other blood cells. These plasma cells are responsible for the secretion of antibodies in the organism. 

 

Recently, it has been shown that extracellular vesicles (EVs) of multiple myeloma cells play a major role in the progress of the pathology. Inside these EVs are found several markers linked to this progression, among which the neurotrophin receptor TRKC and its co-receptor sortilin as described by the CoMeT laboratory. 

 

Up to now, analysis of EVs markers was made on full EVs preparations by successive fluorescent detection of EVs markers or global detection by western blotting. Henceforth, it is necessary to characterize more precisely each type of EVs by simultaneous multi-marking.

The internship will consist in perfecting another method of characterization using spectral flow cytometry. Thanks to this technology, it will be possible to characterize, at the same time, many vesicle markers of an entire preparation or markers of interest (TRKC, sortilin, Cx43…) for each EV sample.